pCMV-Tag2B EDD

LOCUS       pCMV-Tag2B EDD               12771 bp ds-DNA     circular SYN 01-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Tag2B EDD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 1, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             616..639
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9100..9117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9134..9150)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9135..9151)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9206..9225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9207..9225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    9499..9620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9536..9555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9590..9609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9627..10082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9764..9785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9976..9995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10109..10213
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10215..10572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(10238..10258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10423..10558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10485..10504
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10607..11401
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10661..10680)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11271..11290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11589..11608)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11633..11680
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12009..12597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12498..12517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      601 gcggccgcca ccatggatta caaggatgac gacgataaga gcccgggcgg atcccccggg
      661 ctgcaggaat tcgatcgagt ggaggacgag aaggaaagca ccatgacgtc catccatttc
      721 gtggttcacc cgctgccggg caccgaggac cagctcaatg acaggttacg agaagtttct
      781 gagaagctga acaaatataa tttaaacagc cacccccctt tgaatgtatt ggaacaggct
      841 actattaaac agtgtgtggt gggaccaaat catgctgcct ttcttcttga ggatggtaga
      901 gtttgcagga ttggtttttc agtacagcca gacagattgg aattgggtaa acctgataat
      961 aatgatgggt caaagttgaa cagcaactcg ggggcaggga ggacgtcaag gcctggtagg
     1021 acaagcgact ctccatggtt tctctcaggt tctgagactc taggcaggct ggcaggcaac
     1081 accttaggaa gccgctggag ttctggagtg ggtggaagtg gtggaggatc ctctggtagg
     1141 tcatcagctg gagctcgaga ttcccgccgg cagactcgag ttattcggac aggacgggat
     1201 cgagggtctg ggcttttggg cagtcagccc cagccagtta ttccagcatc tgtcattcca
     1261 gaggagctga tttcacaggc ccaagttgtt ttacaaggca aatccagaag tgtcattatt
     1321 cgagaacttc agagaacaaa tcttgatgtg aaccttgctg taaataattt acttagccgg
     1381 gatgatgaag atggagatga tggggatgat acagccagcg aatcttattt gcctggagag
     1441 gatcttatgt ctctccttga tgccgacatt cattctgccc acccaagtgt cattattgat
     1501 gcagatgcca tgttttctga agacattagc tattttggtt acccttcttt tcgtcgttca
     1561 tcactttcca ggctaggctc atctcgagtt ctccttcttc ccttagagag agactctgag
     1621 ctgttgcgtg aacgtgaatc cgttttacgt ttacgtgaac gaaggtggct tgatggagcc
     1681 tcatttgata atgaaagggg ttctaccagc aaggaaggag agccaaactt ggataagaag
     1741 aatacacctg ttcaaagtcc agtatctcta ggagaagatt tgcagtggtg gcctgataag
     1801 gatggaacaa aattcatctg tattggggct ctgtattctg aacttctggc tgtcagcagt
     1861 aaaggagaac tttatcagtg gaaatggagt gaatctgagc cttacagaaa tgcccagaat
     1921 ccttcattac atcatccacg agcaacattt ttggggttaa ccaatgaaaa gatagtcctc
     1981 ctgtctgcaa atagcataag agcaactgta gctacagaaa ataacaaggt tgctacatgg
     2041 gtggatgaaa ctttaagttc tgtggcttct aaattagagc acactgctca gacttactct
     2101 gaacttcaag gagagcggat agtttcttta cattgctgtg ccctttacac ctgcgctcag
     2161 ctggaaaaca gtttatattg gtggggtgta gttcctttta gtcaaaggag gaaaatgtta
     2221 gagaaagcta gagcaaaaaa taaaaagcct aaatccagtg ctggtatttc ttcaatgccg
     2281 aacatcactg ttggtaccca ggtatgcttg agaaataatc ctctttatca tgctggagca
     2341 gttgcatttt caattagtgc tgggattcct aaagttggtg tcttaatgga gtcagtttgg
     2401 aatatgaatg acagctgtag atttcaactt agatctcctg aaagcttgaa aaacatggaa
     2461 aaagctagca aaactactga agctaagcct gaaagtaagc aggagccagt gaaaacagaa
     2521 atgggtcctc caccatctcc agcatccacg tgtagtgatg catcctcaat tgccagcagt
     2581 gcatcaatgc catacaaacg acgacggtca acccctgcac caaaagaaga ggaaaaggtg
     2641 aatgaagagc agtggtctct tcgggaagtg gtttttgtgg aagatgtcaa gaatgttcct
     2701 gttggcaagg tgctaaaagt agatggtgcc tatgttgctg taaaatttcc aggaacctcc
     2761 agtaatacta actgtcagaa cagctctggt ccagatgctg acccttcttc tctcctgcag
     2821 gattgtaggt tacttagaat tgatgaattg caggttgtca aaactggtgg aacaccgaag
     2881 gttcccgact gtttccaaag gactcctaaa aagctttgta tacctgaaaa aacagaaata
     2941 ttagcagtga atgtagattc caaaggtgtt catgctgttc tgaagactgg aaattgggta
     3001 cgatactgta tctttgatct tgctacagga aaagcagaac aggaaaataa ttttcctaca
     3061 agcagcattg ctttccttgg tcagaatgag aggaatgtag ccattttcac tgctggacag
     3121 gaatctccca ttattcttcg agatggaaat ggtaccatct acccaatggc caaagattgc
     3181 atgggaggaa taagggatcc cgattggctg gatcttccac ctattagtag tcttggaatg
     3241 ggtgtgcatt ctttaataaa tcttcctgcc aattcaacaa tcaaaaagaa agctgctgtt
     3301 atcatcatgg ctgtagagaa acaaacctta atgcaacaca ttctgcgctg tgactatgag
     3361 gcctgtcgac aatatctaat gaatcttgag caagcggttg ttttagagca gaatctacag
     3421 atgctgcaga cattcatcag ccacagatgt gatggaaatc gaaatatttt gcatgcttgt
     3481 gtatcagttt gctttccaac cagcaataaa gaaactaaag aagaagagga agcggagcgt
     3541 tctgaaagaa atacatttgc agaaaggctt tctgctgttg aggccattgc aaatgcaata
     3601 tcagttgttt caagtaatgg cccaggtaat cgggctggat catcaagtag ccgaagtttg
     3661 agattacggg aaatgatgag acgttcgttg agagcagctg gtttgggtag acatgaagct
     3721 ggagcttcat ccagtgacca ccaggatcca gtttcacccc ccatagctcc ccctagttgg
     3781 gttcctgacc ctcctgcgat ggatcctgat ggtgacattg attttatcct ggcccccgct
     3841 gtgggatctc ttaccacagc agcaaccggt actggtcaag gaccaagcac ctccactatt
     3901 ccaggtcctt ccacagagcc atctgtagta gaatccaagg atcgaaaggc gaatgctcat
     3961 tttatattga aattgttatg tgacagtgtg gttctccagc cctatctacg agaacttctt
     4021 tctgccaagg atgcaagagg gatgacccca tttatgtcag ctgtaagtgg ccgagcttat
     4081 cctgctgcaa ttaccatctt agaaactgct cagaaaattg caaaagctga aatatcctca
     4141 agtgaaaaag aggaagatgt attcatggga atggtttgcc catcaggtac caaccctgat
     4201 gactctcctt tatatgtttt atgttgtaat gacacttgca gttttacatg gactggagca
     4261 gagcacatta accaggatat ttttgagtgt cgaacttgtg gcttgctgga gtcactgtgt
     4321 tgttgtacgg aatgtgcaag ggtttgtcat aaaggtcatg attgcaaact caaacggaca
     4381 tcaccaacag cctactgtga ttgttgggag aaatgtaaat gtaaaactct tattgctgga
     4441 cagaaatctg ctcgtcttga tctactttat cgcctgctca ctgctactaa tctggttact
     4501 ctgccaaaca gcaggggaga gcacctctta ctattcttag tacagacagt cgcaaggcag
     4561 acggtggagc attgtcaata caggccacct cgaatcaggg aagatcgtaa ccgaaaaaca
     4621 gccagtcctg aagattcaga tatgccagat catgatttgg agcctccaag atttgcccag
     4681 cttgcattgg agcgtgttct acaggactgg aatgccttga aatctatgat tatgtttggg
     4741 tcgcaggaga ataaagaccc tcttagtgcc agcagtagaa taggccatct tttgccagaa
     4801 gagcaagtat acctcaatca gcaaagtggc acaattcggc tggactgttt cactcattgc
     4861 cttatagtta agtgtacagc agatattttg cttttagata ctctactagg tacactagtg
     4921 aaagaactcc aaaacaaata tacacctgga cgtagagaag aagctattgc tgtgacaatg
     4981 aggtttctac gttcagtggc aagagttttt gttattctga gtgtggaaat ggcttcatcc
     5041 aaaaagaaaa acaactttat tccacagcca attggaaaat gcaagcgtgt attccaagca
     5101 ttgctacctt acgctgtgga agaattgtgc aacgtagcag agtcactgat tgttcctgtc
     5161 agaatgggga ttgctcgtcc aactgcacca tttaccctgg ctagtactag catagatgcc
     5221 atgcagggca gtgaagaatt attttcagtg gaaccactac caccacgacc atcatctgat
     5281 cagtctagca gctccagtca gtctcagtca tcctacatca tcaggaatcc acagcagagg
     5341 cgcatcagcc agtcacagcc cgttcggggc agagatgaag aacaggatga tattgtttca
     5401 gcagatgtgg aagaggttga ggtggtggag ggtgtggctg gagaagagga tcatcatgat
     5461 gaacaggaag aacacgggga agaaaatgct gaggcagagg gacaacatga tgagcatgat
     5521 gaagacggga gtgatatgga gctggacttg ttagcagcag ctgaaacaga aagtgatagt
     5581 gaaagtaacc acagcaacca agataatgct agtgggcgca gaagcgttgt cactgcagca
     5641 actgctggtt cagaagcagg agcaagcagt gttcctgcct tcttttctga agatgattct
     5701 caatcgaatg actcaagtga ttctgatagc agtagtagtc agagtgacga catagaacag
     5761 gagaccttta tgcttgatga gccattagaa agaaccacaa atagctccca tgccaatggt
     5821 gctgcccaag ctccccgttc aatgcagtgg gctgtccgca acacccagca tcagcgagca
     5881 gccagtacag ccccttccag tacatctaca ccagcagcaa gttcagcggg tttgatttat
     5941 attgatcctt caaacttacg ccggagtggt accatcagta caagtgctgc agctgcagca
     6001 gctgctttgg aagctagcaa cgccagcagt tacctaacat ctgcaagcag tttagccagg
     6061 gcttacagca ttgtcattag acaaatctcg gacttgatgg gccttattcc taagtataat
     6121 cacctagtat actctcagat tccagcagct gtgaaattga cttaccaaga tgcagtaaac
     6181 ttacagaact atgtagaaga aaagcttatt cccacttgga actggatggt cagtattatg
     6241 gattctactg aagctcaatt acgttatggt tctgcattag catctgctgg tgatcctgga
     6301 catccaaatc atcctcttca cgcttctcag aattcagcga gaagagagag gatgactgcg
     6361 cgagaagaag ctagcttacg aacacttgaa ggcagacgac gtgccacctt gcttagcgcc
     6421 cgtcaaggaa tgatgtctgc acgaggagac ttcctaaatt atgctctgtc tctaatgcgg
     6481 tctcataatg atgagcattc tgatgttctt ccagttttgg atgtttgctc attgaagcat
     6541 gtggcatatg tttttcaagc acttatatac tggattaagg caatgaatca gcagacaaca
     6601 ttggatacac ctcaactaga acgcaaaagg acgcgagaac tcttggaact gggtattgat
     6661 aatgaagatt cagaacatga aaatgatgat gacaccaatc aaagtgctac tttgaatgat
     6721 aaggatgatg actctcttcc tgcagaaact ggccaaaacc atccattttt ccgacgttca
     6781 gactccatga cattccttgg gtgtataccc ccaaatccat ttgaagtgcc tctggctgaa
     6841 gccatcccct tggctgatca gccacatctg ttgcagccaa atgctagaaa ggaggatctt
     6901 tttggccgtc caagtcaggg tctttattct tcatctgcca gtagtgggaa atgtttaatg
     6961 gaggttacag tggatagaaa ctgcctagag gttcttccaa caaaaatgtc ttatgctgcc
     7021 aatctgaaaa atgtaatgaa catgcaaaac cggcaaaaaa aagaagggga agaacagccc
     7081 gtgctgccag aagaaactga gagttcaaaa ccagggccat ctgctcatga tcttgctgca
     7141 caattaaaaa gtagcttact agcagaaata ggacttactg aaagtgaagg gccacctctc
     7201 acatctttca ggccacagtg tagctttatg ggaatggtta tttcccatga tatgctgcta
     7261 ggacgttggc gcctttcttt agaactgttc ggcagggtat tcatggaaga tgttggagca
     7321 gaacctggat caatcctaac tgaattgggt ggttttgagg taaaagaatc aaaattccgc
     7381 agagaaatgg aaaaactgag aaaccagcag tcaagagatt tgtcactaga ggttgatcgg
     7441 gatcgagatc ttctcattca gcagactatg aggcagctta acaatcactt tggtcgaaga
     7501 tgtgctacta caccaatggc tgtacacaga gtaaaagtca catttaagga tgagccagga
     7561 gagggcagtg gtgtagcacg aagtttttat acagccattg cacaagcatt tttatcaaat
     7621 gaaaaattgc caaatctaga gtgtatccaa aatgccaaca aaggcaccca cacaagttta
     7681 atgcagagat taaggaaccg aggagagaga gaccgggaaa gggagagaga aagggaaatg
     7741 aggaggagta gtggtttgcg agcaggttct cggagggacc gggatagaga ctttagaaga
     7801 cagctttcca tcgacactag gccctttaga ccagcctctg aagggaatcc tagcgatgat
     7861 cctgagcctt tgccagcaca tcggcaggca cttggagaga ggctttatcc tcgtgtacaa
     7921 gcaatgcaac cagcatttgc aagtaaaatc actggcatgt tgttggaatt atccccagct
     7981 cagctgcttc tccttctagc aagtgaggat tctctgagag caagagtgga tgaggccatg
     8041 gaactcatta ttgcacatgg acgggaaaat ggagctgata gtatcctgga tcttggatta
     8101 gtagactcct cagaaaaggt acagcaggaa aaccgaaagc gccatggctc tagtcgaagt
     8161 gtagtagata tggatttaga tgatacagat gatggtgatg acaatgcccc tttgttttac
     8221 caacctggga aaagaggatt ttatactcca aggcctggca agaacacaga agcaaggttg
     8281 aattgtttca gaaacattgg caggattctt ggactatgtc tgttacagaa tgaactctgt
     8341 cctatcacat tgaatagaca tgtaattaaa gtattgcttg gtagaaaagt caattggcat
     8401 gattttgctt tttttgatcc tgtaatgtat gagagtttgc ggcaactaat cctcgcgtct
     8461 cagagttcag atgctgatgc tgttttctca gcaatggatt tggcatttgc aattgacctg
     8521 tgtaaagaag aaggtggagg acaggttgaa ctcattccta atggtgtaaa tataccagtc
     8581 actccacaga atgtatatga gtatgtgcgg aaatacgcag aacacagaat gttggtagtt
     8641 gcagaacagc ccttacatgc aatgaggaaa ggtctactag atgtgcttcc aaaaaattca
     8701 ttagaagatt taacggcaga agattttagg cttttggtaa atggctgcgg tgaagtcaat
     8761 gtgcaaatgc tgatcagttt tacctctttc aatgatgaat caggagaaaa tgctgagaag
     8821 cttctgcagt tcaagcgttg gttctggtca atagtagaga agatgagcat gacagaacga
     8881 caagatcttg tttacttttg gacatcaagc ccatcactgc cagccagtga agaaggattc
     8941 cagcctatgc cctcaatcac aataagacca ccagatgacc aacatcttcc tactgcaaat
     9001 acttgcattt ctcgacttta cgtcccactc tattcctcta aacagattct caaacagaaa
     9061 ttgttactcg ccattaagac caagaatttt ggttttgtgc atcaccatca ccatcactag
     9121 ccatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacctta attaattaag
     9181 gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt cactcgatcg
     9241 cccttcccaa cagttgcgca gcctgaatgg cgaatggaga tccaattttt aagtgtataa
     9301 tgtgttaaac tactgattct aattgtttgt gtattttaga ttcacagtcc caaggctcat
     9361 ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta
     9421 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9481 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9601 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     9661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     9721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     9781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     9841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    10021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    10081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    10141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    10201 aaaaaggaag aatcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
    10261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10321 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    10381 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    10441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10561 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
    10621 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
    10681 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
    10741 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
    10801 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10861 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10921 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10981 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    11041 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagaac atcaggggct
    11101 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
    11161 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    11221 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    11281 ccgtgatatt gctgaagaac ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    11341 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    11401 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    11461 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    11521 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
    11581 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    11641 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
    11701 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
    11761 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
    11821 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
    11881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    12001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    12061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    12121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    12181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    12241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    12301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    12361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    12421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    12481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    12541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    12601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    12661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
    12721 atagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//