| 质粒名称: | pCMV-Tag2B EDD |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 37188 |
| 存储实验室: | Darren Saunders, Charles Watts |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-Tag2B |
| 载体出品公司: | Stratagene |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4373 bp |
| 完整质粒大小: | 12770 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EDD |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 8397 bp |
| 基因突变: | UBR5 (a.k.a. DD5, EDD, EDD1, HYD) |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
FLAG (N terminal on insert)
6x His Tag (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ApaI(未破坏) |
| 3' 克隆位点: | SacII(未破坏) |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | T7 Forward |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Tag2B EDD was a gift from Darren Saunders & Charles Watts (Addgene plasmid # 37188 ; http://n2t.net/addgene:37188 ; RRID:Addgene_37188) |
| 参考文献部分: | EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response. Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK. J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13. 10.1074/jbc.M203527200 PubMed 12011095 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Tag2B EDD 12771 bp ds-DNA circular SYN 01-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV-Tag2B EDD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12771)
AUTHORS Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM,
Calanni ST, Jans DA, Sutherland RL, Watts CK
TITLE EDD, the human hyperplastic discs protein, has a role in
progesterone receptor coactivation and potential involvement in DNA
damage response.
JOURNAL J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
PUBMED 12011095
REFERENCE 2 (bases 1 to 12771)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 1, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12771
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter 554..572
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 616..639
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 9100..9117
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(9134..9150)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9135..9151)
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind complement(9206..9225)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9207..9225)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 9499..9620
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9536..9555)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9590..9609
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(9627..10082)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9764..9785)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9976..9995
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 10109..10213
/gene="bla"
/label=AmpR promoter
promoter 10215..10572
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(10238..10258)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 10423..10558
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10485..10504
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10607..11401
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10661..10680)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 11271..11290
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(11589..11608)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 11633..11680
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 12009..12597
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 12498..12517
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
601 gcggccgcca ccatggatta caaggatgac gacgataaga gcccgggcgg atcccccggg
661 ctgcaggaat tcgatcgagt ggaggacgag aaggaaagca ccatgacgtc catccatttc
721 gtggttcacc cgctgccggg caccgaggac cagctcaatg acaggttacg agaagtttct
781 gagaagctga acaaatataa tttaaacagc cacccccctt tgaatgtatt ggaacaggct
841 actattaaac agtgtgtggt gggaccaaat catgctgcct ttcttcttga ggatggtaga
901 gtttgcagga ttggtttttc agtacagcca gacagattgg aattgggtaa acctgataat
961 aatgatgggt caaagttgaa cagcaactcg ggggcaggga ggacgtcaag gcctggtagg
1021 acaagcgact ctccatggtt tctctcaggt tctgagactc taggcaggct ggcaggcaac
1081 accttaggaa gccgctggag ttctggagtg ggtggaagtg gtggaggatc ctctggtagg
1141 tcatcagctg gagctcgaga ttcccgccgg cagactcgag ttattcggac aggacgggat
1201 cgagggtctg ggcttttggg cagtcagccc cagccagtta ttccagcatc tgtcattcca
1261 gaggagctga tttcacaggc ccaagttgtt ttacaaggca aatccagaag tgtcattatt
1321 cgagaacttc agagaacaaa tcttgatgtg aaccttgctg taaataattt acttagccgg
1381 gatgatgaag atggagatga tggggatgat acagccagcg aatcttattt gcctggagag
1441 gatcttatgt ctctccttga tgccgacatt cattctgccc acccaagtgt cattattgat
1501 gcagatgcca tgttttctga agacattagc tattttggtt acccttcttt tcgtcgttca
1561 tcactttcca ggctaggctc atctcgagtt ctccttcttc ccttagagag agactctgag
1621 ctgttgcgtg aacgtgaatc cgttttacgt ttacgtgaac gaaggtggct tgatggagcc
1681 tcatttgata atgaaagggg ttctaccagc aaggaaggag agccaaactt ggataagaag
1741 aatacacctg ttcaaagtcc agtatctcta ggagaagatt tgcagtggtg gcctgataag
1801 gatggaacaa aattcatctg tattggggct ctgtattctg aacttctggc tgtcagcagt
1861 aaaggagaac tttatcagtg gaaatggagt gaatctgagc cttacagaaa tgcccagaat
1921 ccttcattac atcatccacg agcaacattt ttggggttaa ccaatgaaaa gatagtcctc
1981 ctgtctgcaa atagcataag agcaactgta gctacagaaa ataacaaggt tgctacatgg
2041 gtggatgaaa ctttaagttc tgtggcttct aaattagagc acactgctca gacttactct
2101 gaacttcaag gagagcggat agtttcttta cattgctgtg ccctttacac ctgcgctcag
2161 ctggaaaaca gtttatattg gtggggtgta gttcctttta gtcaaaggag gaaaatgtta
2221 gagaaagcta gagcaaaaaa taaaaagcct aaatccagtg ctggtatttc ttcaatgccg
2281 aacatcactg ttggtaccca ggtatgcttg agaaataatc ctctttatca tgctggagca
2341 gttgcatttt caattagtgc tgggattcct aaagttggtg tcttaatgga gtcagtttgg
2401 aatatgaatg acagctgtag atttcaactt agatctcctg aaagcttgaa aaacatggaa
2461 aaagctagca aaactactga agctaagcct gaaagtaagc aggagccagt gaaaacagaa
2521 atgggtcctc caccatctcc agcatccacg tgtagtgatg catcctcaat tgccagcagt
2581 gcatcaatgc catacaaacg acgacggtca acccctgcac caaaagaaga ggaaaaggtg
2641 aatgaagagc agtggtctct tcgggaagtg gtttttgtgg aagatgtcaa gaatgttcct
2701 gttggcaagg tgctaaaagt agatggtgcc tatgttgctg taaaatttcc aggaacctcc
2761 agtaatacta actgtcagaa cagctctggt ccagatgctg acccttcttc tctcctgcag
2821 gattgtaggt tacttagaat tgatgaattg caggttgtca aaactggtgg aacaccgaag
2881 gttcccgact gtttccaaag gactcctaaa aagctttgta tacctgaaaa aacagaaata
2941 ttagcagtga atgtagattc caaaggtgtt catgctgttc tgaagactgg aaattgggta
3001 cgatactgta tctttgatct tgctacagga aaagcagaac aggaaaataa ttttcctaca
3061 agcagcattg ctttccttgg tcagaatgag aggaatgtag ccattttcac tgctggacag
3121 gaatctccca ttattcttcg agatggaaat ggtaccatct acccaatggc caaagattgc
3181 atgggaggaa taagggatcc cgattggctg gatcttccac ctattagtag tcttggaatg
3241 ggtgtgcatt ctttaataaa tcttcctgcc aattcaacaa tcaaaaagaa agctgctgtt
3301 atcatcatgg ctgtagagaa acaaacctta atgcaacaca ttctgcgctg tgactatgag
3361 gcctgtcgac aatatctaat gaatcttgag caagcggttg ttttagagca gaatctacag
3421 atgctgcaga cattcatcag ccacagatgt gatggaaatc gaaatatttt gcatgcttgt
3481 gtatcagttt gctttccaac cagcaataaa gaaactaaag aagaagagga agcggagcgt
3541 tctgaaagaa atacatttgc agaaaggctt tctgctgttg aggccattgc aaatgcaata
3601 tcagttgttt caagtaatgg cccaggtaat cgggctggat catcaagtag ccgaagtttg
3661 agattacggg aaatgatgag acgttcgttg agagcagctg gtttgggtag acatgaagct
3721 ggagcttcat ccagtgacca ccaggatcca gtttcacccc ccatagctcc ccctagttgg
3781 gttcctgacc ctcctgcgat ggatcctgat ggtgacattg attttatcct ggcccccgct
3841 gtgggatctc ttaccacagc agcaaccggt actggtcaag gaccaagcac ctccactatt
3901 ccaggtcctt ccacagagcc atctgtagta gaatccaagg atcgaaaggc gaatgctcat
3961 tttatattga aattgttatg tgacagtgtg gttctccagc cctatctacg agaacttctt
4021 tctgccaagg atgcaagagg gatgacccca tttatgtcag ctgtaagtgg ccgagcttat
4081 cctgctgcaa ttaccatctt agaaactgct cagaaaattg caaaagctga aatatcctca
4141 agtgaaaaag aggaagatgt attcatggga atggtttgcc catcaggtac caaccctgat
4201 gactctcctt tatatgtttt atgttgtaat gacacttgca gttttacatg gactggagca
4261 gagcacatta accaggatat ttttgagtgt cgaacttgtg gcttgctgga gtcactgtgt
4321 tgttgtacgg aatgtgcaag ggtttgtcat aaaggtcatg attgcaaact caaacggaca
4381 tcaccaacag cctactgtga ttgttgggag aaatgtaaat gtaaaactct tattgctgga
4441 cagaaatctg ctcgtcttga tctactttat cgcctgctca ctgctactaa tctggttact
4501 ctgccaaaca gcaggggaga gcacctctta ctattcttag tacagacagt cgcaaggcag
4561 acggtggagc attgtcaata caggccacct cgaatcaggg aagatcgtaa ccgaaaaaca
4621 gccagtcctg aagattcaga tatgccagat catgatttgg agcctccaag atttgcccag
4681 cttgcattgg agcgtgttct acaggactgg aatgccttga aatctatgat tatgtttggg
4741 tcgcaggaga ataaagaccc tcttagtgcc agcagtagaa taggccatct tttgccagaa
4801 gagcaagtat acctcaatca gcaaagtggc acaattcggc tggactgttt cactcattgc
4861 cttatagtta agtgtacagc agatattttg cttttagata ctctactagg tacactagtg
4921 aaagaactcc aaaacaaata tacacctgga cgtagagaag aagctattgc tgtgacaatg
4981 aggtttctac gttcagtggc aagagttttt gttattctga gtgtggaaat ggcttcatcc
5041 aaaaagaaaa acaactttat tccacagcca attggaaaat gcaagcgtgt attccaagca
5101 ttgctacctt acgctgtgga agaattgtgc aacgtagcag agtcactgat tgttcctgtc
5161 agaatgggga ttgctcgtcc aactgcacca tttaccctgg ctagtactag catagatgcc
5221 atgcagggca gtgaagaatt attttcagtg gaaccactac caccacgacc atcatctgat
5281 cagtctagca gctccagtca gtctcagtca tcctacatca tcaggaatcc acagcagagg
5341 cgcatcagcc agtcacagcc cgttcggggc agagatgaag aacaggatga tattgtttca
5401 gcagatgtgg aagaggttga ggtggtggag ggtgtggctg gagaagagga tcatcatgat
5461 gaacaggaag aacacgggga agaaaatgct gaggcagagg gacaacatga tgagcatgat
5521 gaagacggga gtgatatgga gctggacttg ttagcagcag ctgaaacaga aagtgatagt
5581 gaaagtaacc acagcaacca agataatgct agtgggcgca gaagcgttgt cactgcagca
5641 actgctggtt cagaagcagg agcaagcagt gttcctgcct tcttttctga agatgattct
5701 caatcgaatg actcaagtga ttctgatagc agtagtagtc agagtgacga catagaacag
5761 gagaccttta tgcttgatga gccattagaa agaaccacaa atagctccca tgccaatggt
5821 gctgcccaag ctccccgttc aatgcagtgg gctgtccgca acacccagca tcagcgagca
5881 gccagtacag ccccttccag tacatctaca ccagcagcaa gttcagcggg tttgatttat
5941 attgatcctt caaacttacg ccggagtggt accatcagta caagtgctgc agctgcagca
6001 gctgctttgg aagctagcaa cgccagcagt tacctaacat ctgcaagcag tttagccagg
6061 gcttacagca ttgtcattag acaaatctcg gacttgatgg gccttattcc taagtataat
6121 cacctagtat actctcagat tccagcagct gtgaaattga cttaccaaga tgcagtaaac
6181 ttacagaact atgtagaaga aaagcttatt cccacttgga actggatggt cagtattatg
6241 gattctactg aagctcaatt acgttatggt tctgcattag catctgctgg tgatcctgga
6301 catccaaatc atcctcttca cgcttctcag aattcagcga gaagagagag gatgactgcg
6361 cgagaagaag ctagcttacg aacacttgaa ggcagacgac gtgccacctt gcttagcgcc
6421 cgtcaaggaa tgatgtctgc acgaggagac ttcctaaatt atgctctgtc tctaatgcgg
6481 tctcataatg atgagcattc tgatgttctt ccagttttgg atgtttgctc attgaagcat
6541 gtggcatatg tttttcaagc acttatatac tggattaagg caatgaatca gcagacaaca
6601 ttggatacac ctcaactaga acgcaaaagg acgcgagaac tcttggaact gggtattgat
6661 aatgaagatt cagaacatga aaatgatgat gacaccaatc aaagtgctac tttgaatgat
6721 aaggatgatg actctcttcc tgcagaaact ggccaaaacc atccattttt ccgacgttca
6781 gactccatga cattccttgg gtgtataccc ccaaatccat ttgaagtgcc tctggctgaa
6841 gccatcccct tggctgatca gccacatctg ttgcagccaa atgctagaaa ggaggatctt
6901 tttggccgtc caagtcaggg tctttattct tcatctgcca gtagtgggaa atgtttaatg
6961 gaggttacag tggatagaaa ctgcctagag gttcttccaa caaaaatgtc ttatgctgcc
7021 aatctgaaaa atgtaatgaa catgcaaaac cggcaaaaaa aagaagggga agaacagccc
7081 gtgctgccag aagaaactga gagttcaaaa ccagggccat ctgctcatga tcttgctgca
7141 caattaaaaa gtagcttact agcagaaata ggacttactg aaagtgaagg gccacctctc
7201 acatctttca ggccacagtg tagctttatg ggaatggtta tttcccatga tatgctgcta
7261 ggacgttggc gcctttcttt agaactgttc ggcagggtat tcatggaaga tgttggagca
7321 gaacctggat caatcctaac tgaattgggt ggttttgagg taaaagaatc aaaattccgc
7381 agagaaatgg aaaaactgag aaaccagcag tcaagagatt tgtcactaga ggttgatcgg
7441 gatcgagatc ttctcattca gcagactatg aggcagctta acaatcactt tggtcgaaga
7501 tgtgctacta caccaatggc tgtacacaga gtaaaagtca catttaagga tgagccagga
7561 gagggcagtg gtgtagcacg aagtttttat acagccattg cacaagcatt tttatcaaat
7621 gaaaaattgc caaatctaga gtgtatccaa aatgccaaca aaggcaccca cacaagttta
7681 atgcagagat taaggaaccg aggagagaga gaccgggaaa gggagagaga aagggaaatg
7741 aggaggagta gtggtttgcg agcaggttct cggagggacc gggatagaga ctttagaaga
7801 cagctttcca tcgacactag gccctttaga ccagcctctg aagggaatcc tagcgatgat
7861 cctgagcctt tgccagcaca tcggcaggca cttggagaga ggctttatcc tcgtgtacaa
7921 gcaatgcaac cagcatttgc aagtaaaatc actggcatgt tgttggaatt atccccagct
7981 cagctgcttc tccttctagc aagtgaggat tctctgagag caagagtgga tgaggccatg
8041 gaactcatta ttgcacatgg acgggaaaat ggagctgata gtatcctgga tcttggatta
8101 gtagactcct cagaaaaggt acagcaggaa aaccgaaagc gccatggctc tagtcgaagt
8161 gtagtagata tggatttaga tgatacagat gatggtgatg acaatgcccc tttgttttac
8221 caacctggga aaagaggatt ttatactcca aggcctggca agaacacaga agcaaggttg
8281 aattgtttca gaaacattgg caggattctt ggactatgtc tgttacagaa tgaactctgt
8341 cctatcacat tgaatagaca tgtaattaaa gtattgcttg gtagaaaagt caattggcat
8401 gattttgctt tttttgatcc tgtaatgtat gagagtttgc ggcaactaat cctcgcgtct
8461 cagagttcag atgctgatgc tgttttctca gcaatggatt tggcatttgc aattgacctg
8521 tgtaaagaag aaggtggagg acaggttgaa ctcattccta atggtgtaaa tataccagtc
8581 actccacaga atgtatatga gtatgtgcgg aaatacgcag aacacagaat gttggtagtt
8641 gcagaacagc ccttacatgc aatgaggaaa ggtctactag atgtgcttcc aaaaaattca
8701 ttagaagatt taacggcaga agattttagg cttttggtaa atggctgcgg tgaagtcaat
8761 gtgcaaatgc tgatcagttt tacctctttc aatgatgaat caggagaaaa tgctgagaag
8821 cttctgcagt tcaagcgttg gttctggtca atagtagaga agatgagcat gacagaacga
8881 caagatcttg tttacttttg gacatcaagc ccatcactgc cagccagtga agaaggattc
8941 cagcctatgc cctcaatcac aataagacca ccagatgacc aacatcttcc tactgcaaat
9001 acttgcattt ctcgacttta cgtcccactc tattcctcta aacagattct caaacagaaa
9061 ttgttactcg ccattaagac caagaatttt ggttttgtgc atcaccatca ccatcactag
9121 ccatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacctta attaattaag
9181 gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt cactcgatcg
9241 cccttcccaa cagttgcgca gcctgaatgg cgaatggaga tccaattttt aagtgtataa
9301 tgtgttaaac tactgattct aattgtttgt gtattttaga ttcacagtcc caaggctcat
9361 ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta
9421 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
9481 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
9541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
9601 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
9661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
9721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
9781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
9841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
9901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
9961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
10021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
10081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
10141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
10201 aaaaaggaag aatcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
10261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
10321 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
10381 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
10441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
10501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
10561 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
10621 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
10681 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
10741 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
10801 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
10861 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
10921 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
10981 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
11041 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagaac atcaggggct
11101 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
11161 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
11221 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
11281 ccgtgatatt gctgaagaac ttggcggcga atgggctgac cgcttcctcg tgctttacgg
11341 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
11401 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
11461 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
11521 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
11581 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
11641 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
11701 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
11761 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
11821 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
11881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
11941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
12001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
12061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
12121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
12181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
12241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
12301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
12361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
12421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
12481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
12541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
12601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
12661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
12721 atagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
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